16volt contributed to over 40 original pieces of music for the film.
In the mid-21st century, an epidemic of organ failures leads to the rise of GeneCo., a company providing transplants at a great price. Those who miss their payments become targets of GeneCo. mercenaries, who repossess the organs. In a world of drug addiction and legalized murder, a sheltered youth (Alexa Vega) seeks a cure for her rare disease as well as information about her family’s mysterious history. Her questions are answered at “The Genetic Opera.”
- Alexa Vega as Shilo Wallace, the primary protagonist; 17 years old, she is confined to her room due to the alleged blood disorder she inherited from her mother, and dreams of exploring the world outside of her bedroom, which she considers her prison. While she initially strives to obey her father’s rules, later in the story, she becomes more and more assertive, attempting to earn her independence. Prior to the story she has often snuck out of her room at night to collect bugs, and shots of her room shows she has an impressive collection.
- Paul Sorvino as Rottissimo “Rotti” Largo, the film’s main antagonist; the ailing founder and president of GeneCo, looking for a worthy heir as his terminal disease progresses. He is a powerful and driven man, having built his GeneCo empire from the ground up, and believes that material wealth is the only real driving force in the world. He is ashamed of his own children, and is the one responsible for Marni’s death, shielding the truth from Nathan as revenge for “stealing” Marni from him.
- Anthony Head as Nathan Wallace, Shilo’s father and a widower, who wrongly believes he was inadvertently responsible for Marni’s death. Nathan doubles as the head Repo Man to repay Rotti for “fixing” the circumstances of Marni’s death with the police. Nathan is a highly skilled assassin who often laments his late night duties, though he appears to have some sadistic tendencies as well.
- Sarah Brightman as Magdalene “Blind Mag” Defoe, Shilo’s godmother and Marni’s best friend. She was born blind, but was given surgically enhanced eyes by GeneCo in exchange for having to work as a GeneCo spokesperson for the rest of her life. She is marked for repossession and is set to deliver her final performance for the company.
- Paris Hilton as Carmela Largo/Amber Sweet, Rotti’s only daughter and the youngest of the Largo children. She is a singer addicted to both surgery and Zydrate, which she obtains illegally from GraveRobber, occasionally in exchange for sex.
- Bill Moseley as Luigi Largo, Rotti’s aggressive, homicidal eldest son. He wields a knife at all times, and is willing to stab others at the slightest provocation.
- Nivek Ogre as Pavi Largo, Rotti’s middle child. Womanizing, narcissistic, vacuous and effeminate, he wears the skinned face of a woman as a mask over his own, which is heavily scarred. Due to the way his face is attached, he is able to change faces on a whim. He has an Italian accent (to conceal his speech impediment, according to Zdunich) and is almost always surrounded by women, usually Genterns (GeneCo’s employees).
- Terrance Zdunich as GraveRobber, a peddler who obtains Zydrate from corpses and has connections to Amber, both sexually and as a dealer. He also seems to be Shilo’s first and only friend. Mysterious and charismatic, he acts as the narrator of the post-apocalyptic world.
- Sarah Power as Marni Wallace, Nathan’s late wife and Shilo’s mother; she was engaged to Rotti until she left him for Nathan. A jealous Rotti poisoned her and framed Nathan for the crime.
- Nancy Long provided Marni’s singing voice for “Chase the Morning”.
Joan Jett makes a cameo appearance playing guitar during the song “Seventeen”. Dean Armstrong, later seen in Saw 3D, appears as the victim during “Thankless Job”. Co-writer Darren Smith appears during “We Started This Op’ra Shit” as the GeneCo band leader. Frequent Bousman collaborator J. LaRose appears during “Zydrate Support Network” as a GeneCo spokesman.